Journal: Heliyon
Article Title: Decidual production of interferon lambda in response to ZIKV persistence: Clinical evidence and in vitro modelling
doi: 10.1016/j.heliyon.2024.e30613
Figure Lengend Snippet: ZIKV positivity in at-term placenta modulates transcriptional profile of IFN and inflammation-related genes. Detailed boxplots for log2 normalized expression comparing at-term placenta from healthy controls (n = 10), and placentas from pregnancies with Zika (n = 65), further divided into ZIKV persistent (+) or absent (−) (n = 18 and 47, respectively). Genes analyzed were related to IFN – (A) IFNL1 , (B) IFNL2 , (C) IFNL3 , (D) IFNL4 , (E) IFNA1 , (F) IFNB , and (G) IFIT5 , and inflammation – (H) CCR2 , (I) CCR3 , (J) CCR5 , (K) CXCL10, (L) TNFSF9 , (M) IL10 , (N) IL18 , (O) IL2 2RA, (P) MMP2 , and (Q) AIM2 . Each dot corresponds to one placenta analyzed. Comparisons using Kruskal-Wallis with Dunn test P-values ** ≤0.01, * ≤0.05.
Article Snippet: For IFNL1-4 gene expression, TaqManTM probes (Thermo Fisher Scientific, Waltham, MA, USA) were used according to the manufacturer's instructions (respective assay IDs: Hs00601677_g1, Hs00820125_g1, Hs04193049_gH, and Hs04400217_g1) using 18S (assay ID Hs03003631_g1) as endogenous control.
Techniques: Expressing